Ambiviricota

Ambiviricota is a phylum of ambisense, single-stranded RNA viruses that infect fungi. These RNA virus genomes contain at least two open reading frames in a non-overlapping ambisense orientation. The ambisense structure allows the virus to encode two proteins on opposite strands of RNA. Ambivirus genomes replicate using a rolling-circle mechanism and form a rod-like structure containing ribozymes in both sense and antisense orientations, similar to viroids—small, circular infectious agents that also utilize ribozymes.